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Question
What is the net ATP yield from one glucose molecule in aerobic respiration?
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Answer
30–32 ATP (2 glycolysis + 2 pyruvate decarboxylation + 6 NADH × ~2.5 + 2 FADH₂ × ~1.5 + 2 substrate-level in TCA)
Question
Which amino acids are positively charged at physiological pH?
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Answer
Lysine (Lys, K), Arginine (Arg, R), and Histidine (His, H) — remember "KAR" (K, R, H)
Question
What is the Henderson-Hasselbalch equation?
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Answer
pH = pKa + log([A⁻]/[HA]). Used to calculate the pH of a buffer solution given the concentrations of the weak acid and conjugate base.
Question
Define competitive inhibition.
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Answer
An inhibitor structurally similar to the substrate binds the active site, increasing apparent Km without changing Vmax. Overcome by increasing substrate concentration.
Question
What are the stages of mitosis in order?
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Answer
Prophase → Metaphase → Anaphase → Telophase (PMAT). Chromosomes condense (P), align at metaphase plate (M), sister chromatids separate (A), nuclear envelopes reform (T).
Question
What is the role of the sodium-potassium pump?
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Answer
Pumps 3 Na⁺ out and 2 K⁺ in per ATP hydrolyzed (antiporter). Maintains resting membrane potential (~−70 mV) and cell volume.
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